Western Blotting: Difference between revisions
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Western Blotting (also known as [[Immunoblotting|immunoblotting]]) is a technique used in biomedicine to isolate and identify a [[Protein|protein]] of interest. Western Blotting involves the use of [[Antibody|antibodies]] to bind to the [[Protein|protein]] ([[Primary antibody|primary antibodies]]) and then to identify through [[Bioluminescence|bioluminescence]] ([[Secondary antibody|secondary antibodies]]). | Western Blotting (also known as [[Immunoblotting|immunoblotting]]) is a technique used in biomedicine to isolate and identify a [[Protein|protein]] of interest. Western Blotting involves the use of [[Antibody|antibodies]] to bind to the [[Protein|protein]] ([[Primary antibody|primary antibodies]]) and then to identify through [[Bioluminescence|bioluminescence]] ([[Secondary antibody|secondary antibodies]]). It is possible to not only identify the protein with the fluroescent label but we can also determine the quantity of the protein. | ||
Protocol for Western Blotting: | |||
1.Prepare Tissue | |||
2. Electrophoresis | |||
3. Transfer to polymer sheet | |||
4. Add Antibodies | |||
5. Identify | |||
Diagnosis of Hepatitis C involves the evaluation of a western blot. | |||
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Revision as of 11:02, 21 October 2012
Western Blotting (also known as immunoblotting) is a technique used in biomedicine to isolate and identify a protein of interest. Western Blotting involves the use of antibodies to bind to the protein (primary antibodies) and then to identify through bioluminescence (secondary antibodies). It is possible to not only identify the protein with the fluroescent label but we can also determine the quantity of the protein.
Protocol for Western Blotting:
1.Prepare Tissue
2. Electrophoresis
3. Transfer to polymer sheet
4. Add Antibodies
5. Identify
Diagnosis of Hepatitis C involves the evaluation of a western blot.