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See [[SDS polyacrylamide-gel electrophoresis|SDS polyacrylamide-gel electrophoresis]] | See [[SDS polyacrylamide-gel electrophoresis|SDS polyacrylamide-gel electrophoresis]] | ||
SDS is a negatively charged substance that allows [[ | SDS (Sodium Dodecyl Sulfate) is a negatively charged substance/anionic [[Surfactant|surfactant]] that allows [[Proteins|proteins]] of all charges to travel down the [[Polyacrylamide|polyacrylamide]] to the positive electrode <ref>Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/</ref>. i.e. This SDS will bind to [[Protien|proteins]] and disrupt non-covalent bonds. This causes [[Denature|denaturation]] in proteins. SDS molecule has a [[sulfate|sulfate]] group attacted, a protein will then become negatively charged when it is binded to SDS molecule.<br> | ||
=== References === | === References === | ||
Revision as of 13:07, 25 October 2017
See SDS polyacrylamide-gel electrophoresis
SDS (Sodium Dodecyl Sulfate) is a negatively charged substance/anionic surfactant that allows proteins of all charges to travel down the polyacrylamide to the positive electrode [1]. i.e. This SDS will bind to proteins and disrupt non-covalent bonds. This causes denaturation in proteins. SDS molecule has a sulfate group attacted, a protein will then become negatively charged when it is binded to SDS molecule.
References
- ↑ Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/