Deoxynucleotide: Difference between revisions
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A deoxynucleotide in biological terms is a component of [[Deoxyribonucleic_acid|deoxyribonucleic acid]], [[DNA|DNA]]. A deoxynucleotide is made up of an organic base, a [[Ribose|ribose sugar]] and [[phosphate|phosphate]]. [[DNA polymerase|DNA polymerase]] requires deoxynucleotides in their triphosphate (containing three phosphates) form in order for [[DNA synthesis|DNA synthesis]] to take place<ref>groups.molbiosci.northwestern.edu/holmgren/Glossary/Definitions/Def-D/deoxynucleotide.html</ref>. The 4 triphosphates ([[DNTP|dNTPs]]) are [[ATP|ATP]], [[CTP|CTP]], [[GTP|GTP]] and [[TTP|TTP]], one for each organise base – [[adenine|adenine]], [[cytosine|cytosine]], [[guanine|guanine]] and [[thymine|thymine]] relatively. | |||
Deoxynucleotide triphosphates are an essential component of nucleic acid [[molecules|molecules]] and are required in [[PCR|PCR]] (polymerase chain reaction), a technique used in genetics that analyses a short sequence of DNA and used to amplify sections of DNA that are wanted and required<ref>www.medicinenet.com/script/main/art.asp</ref>. No amplified DNA would be able to be made without dNTPs<ref>ink.springer.com/chapter/10.1007/978-1-4020-6241-4_6</ref>. | |||
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Latest revision as of 19:41, 10 December 2018
A deoxynucleotide in biological terms is a component of deoxyribonucleic acid, DNA. A deoxynucleotide is made up of an organic base, a ribose sugar and phosphate. DNA polymerase requires deoxynucleotides in their triphosphate (containing three phosphates) form in order for DNA synthesis to take place[1]. The 4 triphosphates (dNTPs) are ATP, CTP, GTP and TTP, one for each organise base – adenine, cytosine, guanine and thymine relatively.
Deoxynucleotide triphosphates are an essential component of nucleic acid molecules and are required in PCR (polymerase chain reaction), a technique used in genetics that analyses a short sequence of DNA and used to amplify sections of DNA that are wanted and required[2]. No amplified DNA would be able to be made without dNTPs[3].