Polymerase Chain Reaction (PCR): Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Polymerase Chain Reaction (PCR) is a technique used for the amplification and identification of DNA or RNA. | Polymerase Chain Reaction (PCR) is a technique used for the [[amplification|amplification]] and identification of [[DNA|DNA]] or [[RNA|RNA]]. Also see [[mRNA|mRNA]] | ||
PCR has three main stages: | PCR has three main stages: | ||
1. Heat DNA to 95°c to melt the strands | 1. Heat DNA to 95°c to melt the strands | ||
2. Cool to 50 - 65°c to allow primers to anneal | 2. Cool to 50 - 65°c to allow primers to anneal | ||
3. Heat to 72°c to allow elongation | 3. Heat to 72°c to allow [[elongation|elongation]] | ||
The technique was developed by Kary Mulis in 1983 for which he was awarded the Nobel Prize in Chemistry in 1993. | The technique was developed by Kary Mulis in 1983 for which he was awarded the Nobel Prize in Chemistry in 1993. | ||
Revision as of 11:44, 1 November 2010
Polymerase Chain Reaction (PCR) is a technique used for the amplification and identification of DNA or RNA. Also see mRNA
PCR has three main stages:
1. Heat DNA to 95°c to melt the strands
2. Cool to 50 - 65°c to allow primers to anneal
3. Heat to 72°c to allow elongation
The technique was developed by Kary Mulis in 1983 for which he was awarded the Nobel Prize in Chemistry in 1993.
PCR can be done using water baths at varying temperatures.[1]