Restriction enzyme: Difference between revisions
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Restriction bases are usually 4, 6 or 8 bases long. They recognise specific palindromic base sequences in [[DNA|DNA]] and are used to selectively cut DNA at sites known as '[[Restriction site|restriction sites]]'. Different restriction nucleases are obtained and purified from different species of bacteria. These enzymes are made in bacteria to degrade viral DNA. | Restriction bases are usually 4, 6 or 8 bases long. They recognise specific palindromic base sequences in [[DNA|DNA]] and are used to selectively cut DNA at sites known as '[[Restriction site|restriction sites]]'. Different restriction nucleases are obtained and purified from different species of bacteria. These enzymes are made in bacteria to degrade viral DNA. Cutting [[DNA]] with restriction enzymes can produce fragments with either blunt or sticky ends. | ||
Revision as of 19:56, 26 November 2011
Restriction bases are usually 4, 6 or 8 bases long. They recognise specific palindromic base sequences in DNA and are used to selectively cut DNA at sites known as 'restriction sites'. Different restriction nucleases are obtained and purified from different species of bacteria. These enzymes are made in bacteria to degrade viral DNA. Cutting DNA with restriction enzymes can produce fragments with either blunt or sticky ends.