Dna ligase: Difference between revisions
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DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or [[Okazaki fragments|Okazaki fragments]]. It works by repairing breaks in the [[Sugar phosphate backbone|sugar phosphate backbone]] in [[DNA|DNA]] by creating a covalent bond between adjoining [[Nucleotides|nucleotides]] (between the 3’[[ | DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or [[Okazaki fragments|Okazaki fragments]]. It works by repairing breaks in the [[Sugar phosphate backbone|sugar phosphate backbone]] in [[DNA|DNA]] by creating a covalent bond between adjoining [[Nucleotides|nucleotides]] (between the 3’[[Hydroxyl group|hydroxyl group]] of one DNA molecule with the 5’[[Phosphoryl group|phosphoryl group]] of another).<ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones and Bartlett learning (Chapter 2 DNA Structure and Genetic Variation) p440-42</ref><br>In [[Eukaryotes|eukaryotes]] [[DNA ligase I|DNA ligase I]] family members play the major role.The enzyme has a fundamental role in [[Recombinant DNA Technology|genetic engineering]] such as[[Recombinant DNA Technology|recombinant plasmid formation]] or [[PCR|Polymerase Chain Reaction]].<ref>Berg, J.M., Tymoczko, J.L. and Stryer, L. (2012). Biochemistry, 7 th Edition, New York , W.H.Freeman &amp;amp; Co Ltd. p152-153</ref> <br> | ||
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Revision as of 22:41, 20 October 2012
DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or Okazaki fragments. It works by repairing breaks in the sugar phosphate backbone in DNA by creating a covalent bond between adjoining nucleotides (between the 3’hydroxyl group of one DNA molecule with the 5’phosphoryl group of another).[1]
In eukaryotes DNA ligase I family members play the major role.The enzyme has a fundamental role in genetic engineering such asrecombinant plasmid formation or Polymerase Chain Reaction.[2]
References
- ↑ Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones and Bartlett learning (Chapter 2 DNA Structure and Genetic Variation) p440-42
- ↑ Berg, J.M., Tymoczko, J.L. and Stryer, L. (2012). Biochemistry, 7 th Edition, New York , W.H.Freeman &amp; Co Ltd. p152-153