Genomic library: Difference between revisions
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A genomic library can be created by carrying out a digest of the genomic content of an individual [[organism|organism]]. The fragments of overlapping [[DNA|DNA]] are then inserted into a vector such as a [[lambda-phage|lambda-phage]]. The [[vector|vector]] can then infect a bacteria suh as ''[[E._coli|E. coli]]'' which gives rise to a collection of recombinant clones representing all of the DNA in an organism<ref>Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry. 7th edition. New York: W.H. Freeman and Company . Page 158.</ref>. <br> | |||
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=== References === | === References === | ||
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Latest revision as of 09:29, 22 October 2014
A genomic library can be created by carrying out a digest of the genomic content of an individual organism. The fragments of overlapping DNA are then inserted into a vector such as a lambda-phage. The vector can then infect a bacteria suh as E. coli which gives rise to a collection of recombinant clones representing all of the DNA in an organism[1].
References
- ↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry. 7th edition. New York: W.H. Freeman and Company . Page 158.