Vector: Difference between revisions
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A Vector acts as a template for the cloning of a specific gene. This gene needs to be inserted into the vector by recombinant DNA, before the vector can be inserted into a target host cell. <ref>Hartl D and Ruvolo M (2012). Genetics, Analysis of Genes and Genomes. 8th Edition. United States. Jones and Bartlett Learning.</ref> | A Vector acts as a template for the cloning of a specific gene. This gene needs to be inserted into the vector by recombinant DNA, before the vector can be inserted into a target host cell. <ref>Hartl D and Ruvolo M (2012). Genetics, Analysis of Genes and Genomes. 8th Edition. United States. Jones and Bartlett Learning.</ref> | ||
Vectors are essential components for recombinant [[DNA|DNA]] technology. They act as the vehicle to carry [[Recombinant DNA Technology|recombinant DNA]] into the target host [[Cell|cell]]. | Vectors are essential components for recombinant [[DNA|DNA]] technology. They act as the vehicle to carry [[Recombinant DNA Technology|recombinant DNA]] into the target host [[Cell|cell]]. | ||
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Vectors have special restriction sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient origin of replication. | Vectors have special restriction sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient origin of replication. | ||
The most common types of vectors are a [[Plasmid|plasmid]] (which are circular pieces of DNA naturally found in bacteria) <ref>Genetics: analysis of genes and genomes (2009), Hartl &amp; Jones, 7th Ed. Jones &amp; Bartlett</ref>, viruses and cosmids (a plasmid containing a cos site). <br> | The most common types of vectors are a [[Plasmid|plasmid]] (which are circular pieces of DNA naturally found in bacteria) <ref>Genetics: analysis of genes and genomes (2009), Hartl &amp;amp; Jones, 7th Ed. Jones &amp;amp; Bartlett</ref>, viruses and [[Cosmid|cosmids]] (a plasmid containing a [[cos site|cos site]]). <br> | ||
=== References === | === References === | ||
Revision as of 07:48, 17 October 2015
A Vector acts as a template for the cloning of a specific gene. This gene needs to be inserted into the vector by recombinant DNA, before the vector can be inserted into a target host cell. [1]
Vectors are essential components for recombinant DNA technology. They act as the vehicle to carry recombinant DNA into the target host cell.
Vectors have special restriction sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient origin of replication.
The most common types of vectors are a plasmid (which are circular pieces of DNA naturally found in bacteria) [2], viruses and cosmids (a plasmid containing a cos site).
References