Reporter genes: Difference between revisions
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A reporter [[Gene|gene]] is a gene coding a protein molecule that can be easily assayed. The main use of [[Promoter|promoter]] genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is [[Β-galactosidase|beta-galactosidase.]] In experiments within the lab, reporter genes are usually ligated into a [[Plasmid|plasmid vector]] which contains a gene to select against the bacteria that have not taken up the plasmid (such as antibiotic resistance). The [[DNA|DNA]] fragments which have different length sequences of DNA upstream of the [[Transcription start site|transcription start site]]. | A reporter [[Gene|gene]] is a gene coding a protein molecule that can be easily assayed. The main use of [[Promoter|promoter]] genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is [[Β-galactosidase|beta-galactosidase.]] In experiments within the lab, reporter genes are usually ligated into a [[Plasmid|plasmid vector]] which contains a gene to select against the bacteria that have not taken up the plasmid (such as antibiotic resistance). The [[DNA|DNA]] fragments which have different length sequences of DNA upstream of the [[Transcription start site|transcription start site]]. | ||
The [[Plasmid|plasmids]] are then seperately transfected into cells, such as cultured ''[[Escherichia coli|E. coli]]''. The cell extract can then be easily assayed to show levels of the reporter [[ | The [[Plasmid|plasmids]] are then seperately transfected into cells, such as cultured ''[[Escherichia coli|E. coli]]''. The cell extract can then be easily assayed to show levels of the reporter [[Enzyme|enzyme]] and therefore show how active the [[Promoter|promoter]] is <ref>H. Lodish et al. (2008). Molecular Cell Biology. 8th edition. New York: W.H. Freeman</ref>. | ||
The enhanced green fluorescence protein is as example of a reporter gene. It is derivived from the species, Aequoria victoria (Jellyfish). <ref>This is a ref to the book molecular biology techniques (2012), Carson, Sue Miller, Heather 3 Academic Press 2012, pg2.</ref> | |||
=== References === | === References === | ||
Revision as of 15:54, 20 October 2017
A reporter gene is a gene coding a protein molecule that can be easily assayed. The main use of promoter genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is beta-galactosidase. In experiments within the lab, reporter genes are usually ligated into a plasmid vector which contains a gene to select against the bacteria that have not taken up the plasmid (such as antibiotic resistance). The DNA fragments which have different length sequences of DNA upstream of the transcription start site.
The plasmids are then seperately transfected into cells, such as cultured E. coli. The cell extract can then be easily assayed to show levels of the reporter enzyme and therefore show how active the promoter is [1].
The enhanced green fluorescence protein is as example of a reporter gene. It is derivived from the species, Aequoria victoria (Jellyfish). [2]
References