Morpholino: Difference between revisions
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=== Description === | === Description === | ||
*Small oligomer of synthetic [[ | *Small oligomer of synthetic [[Nucleotide|nucleotide]] analogues that block [[Gene expression|gene expression]]. | ||
*25 bp length | *25 bp length | ||
=== Function === | === Function === | ||
*attaches to mRNA, pre/mature, to cause gene knockdown | *attaches to [[mRNA|mRNA]], pre/mature, to cause [[gene knockdown|gene knockdown]] | ||
*Used as gene knockdown tool on | *Used as gene knockdown tool on Zebra fish<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref> | ||
==== On mature mRNA: ==== | ==== On mature mRNA: ==== | ||
| Line 15: | Line 15: | ||
==== On pre-mRNA: ==== | ==== On pre-mRNA: ==== | ||
*sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking spliceosome which will create truncated/no protein<br> | *sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking [[spliceosome|spliceosome]] which will create truncated/no protein<br> | ||
=== References === | === References === | ||
<references /> | <references /> | ||
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Revision as of 17:16, 16 November 2017
Description
- Small oligomer of synthetic nucleotide analogues that block gene expression.
- 25 bp length
Function
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool on Zebra fish[1]
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking spliceosome which will create truncated/no protein
References
- ↑ Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.