Morpholino: Difference between revisions
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=== Function === | === Function === | ||
*attaches to [[ | *attaches to [[MRNA|mRNA]], pre/mature, to cause [[Gene knockdown|gene knockdown]] | ||
*Used as gene knockdown tool on Zebra fish<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref> | *Used as gene knockdown tool on[[ Zebra fish|Zebra fish]]<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref> | ||
==== On mature mRNA: ==== | ==== On mature mRNA: ==== | ||
*attaches to AUG blocking ribosome interaction | *attaches to AUG blocking [[ribosome|ribosome]] interaction | ||
==== On pre-mRNA: ==== | ==== On pre-mRNA: ==== | ||
*sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking [[ | *sits on splice site, an [[intron-exon junction|intron-exon junction]], making a double-stranded RNA, blocking [[Spliceosome|spliceosome]] which will create truncated/no [[protein|protein]]<br> | ||
=== References === | === References === | ||
Revision as of 17:17, 16 November 2017
Description
- Small oligomer of synthetic nucleotide analogues that block gene expression.
- 25 bp length
Function
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool onZebra fish[1]
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking spliceosome which will create truncated/no protein
References
- ↑ Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.