Non-competative inhibitor: Difference between revisions
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A non-competitive inhibitor is a reversible inhibitor which binds to the [[ | A non-competitive inhibitor is a reversible inhibitor which binds to the [[Enzyme|enzyme]] with weak non-[[Covalent bond|covalent]] interactions. | ||
== Mechanism == | == Mechanism == | ||
Non-competitive inhibition involves the inhibitor binding to an allosteric site on the target [[Enzyme|enzyme]]. This binding causes a conformational change of the [[Enzyme|enzyme]][[Molecule|molecule]] to be altered which in turn causes the shape and conformation of the [[Enzyme active site|active site]] to be changed. Therefore, while the inhibitor is bound to the enzyme, the [[Enzyme|enzyme]] cannot also form the [[Enzyme-substrate complex|enzyme-substrate complex]] which is vital for product formation and thus lowering the activity | Non-competitive inhibition involves the inhibitor binding to an allosteric site on the target [[Enzyme|enzyme]]. This binding causes a conformational change of the [[Enzyme|enzyme]][[Molecule|molecule]] to be altered which in turn causes the shape and conformation of the [[Enzyme active site|active site]] to be changed. Therefore, while the inhibitor is bound to the enzyme, the [[Enzyme|enzyme]] cannot also form the [[Enzyme-substrate complex|enzyme-substrate complex]] which is vital for product formation and thus lowering the activity of the enzyme. | ||
== Effect of inhibitor == | == Effect of inhibitor == | ||
Revision as of 16:24, 21 November 2017
A non-competitive inhibitor is a reversible inhibitor which binds to the enzyme with weak non-covalent interactions.
Mechanism
Non-competitive inhibition involves the inhibitor binding to an allosteric site on the target enzyme. This binding causes a conformational change of the enzymemolecule to be altered which in turn causes the shape and conformation of the active site to be changed. Therefore, while the inhibitor is bound to the enzyme, the enzyme cannot also form the enzyme-substrate complex which is vital for product formation and thus lowering the activity of the enzyme.
Effect of inhibitor
The Km is unchanged as the enzyme still has the same affinity for the substrate molecule.
The Vmax is decreased as the inhibitor prevents the maximum rate of the chemical reaction being reached, therefore decreasing the enzymes efficiency.
Effect of concentration
Non-competitive inhibitors are not affected by changes in substrate concentration, unlike competitive inhibitors.
The extent of inhibition is solely dependent on the concentration of the inhibitor.