Standard curve: Difference between revisions
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Added the references correctly, that is, I added them as explained in the lecture. Cleaned up the text. |
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A Standard curve is a way of plotting data on a graph. It is used to determine relationship between different variables in a | A Standard curve is a way of plotting data on a graph. It is used to determine the relationship between different variables in a dataset. The standard curve is used to determine the value of the unknown variable from the easily measurable variable. | ||
If the procedure follows [[Beer-Lambert Law|Beer's law]], then the plotted points would lie in such a way that a straight line can be drawn through them , this will help to determine the unknown values by locating their %T/Abs on the line then drawing a line to intersect the point on the line and then extending it to the concentration axis< | If the procedure follows [[Beer-Lambert Law|Beer's law]], then the plotted points would lie in such a way that a straight line can be drawn through them, this will help to determine the unknown values by locating their %T/Abs on the line then drawing a line to intersect the point on the line and then extending it to the concentration axis<ref>Thermo Fisher scientific. How to use protein assay standard curve. 2012. [cited: 05/12/17] Available from: https://tools.thermofisher.com/content/sfs/brochures/TR0057-Read-std-curves.pdf </ref>. | ||
the formula for Beer's law is: | the formula for Beer's law is: | ||
A= e x l x c | A= e x l x c | ||
A= Absorbance | A = Absorbance | ||
e = molar absorbtivity constant | |||
l = path length | |||
c = concentration | |||
=== References === | |||
<references /> | |||
Latest revision as of 09:14, 6 December 2017
A Standard curve is a way of plotting data on a graph. It is used to determine the relationship between different variables in a dataset. The standard curve is used to determine the value of the unknown variable from the easily measurable variable.
If the procedure follows Beer's law, then the plotted points would lie in such a way that a straight line can be drawn through them, this will help to determine the unknown values by locating their %T/Abs on the line then drawing a line to intersect the point on the line and then extending it to the concentration axis[1].
the formula for Beer's law is:
A= e x l x c
A = Absorbance
e = molar absorbtivity constant
l = path length
c = concentration
References
- ↑ Thermo Fisher scientific. How to use protein assay standard curve. 2012. [cited: 05/12/17] Available from: https://tools.thermofisher.com/content/sfs/brochures/TR0057-Read-std-curves.pdf