DNA Polymerase: Difference between revisions
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DNA Polymerase is an [[Enzyme|enzyme]] which promotes or catalyzes the joining of [[Deoxyribonucleotide|deoxyribonucleotide]] units ([[Phosphodiester bond|Phosphodiester bond]] formation) to form a [[DNA|DNA]] strand. It is essentially involved in [[DNA|DNA]] replication and [[DNA|DNA]] repair. During [[DNA replication|DNA replication]], DNA polymerase requires the presence of a template [[DNA|DNA]] strand which it copies and synthesizes a new [[DNA|DNA]] strand. The newly synthesized [[DNA|DNA]] strand is complementary to the template strand. The catalytic activity of joining the [[Deoxyribonucleotide|deoxyribonucleotide]] requires four precursors which are the deoxynucleoside 5' triphosphates [[DATP|dATP]], [[DGTP|dGTP]], [[DCTP|dCTP]] and [[DTTP|dTTP]]. [[Magnesium|Magnesium]] [[Ions|ions]] are also required for the catalytic activity <ref>Berg, J. Tymoczko, J. Stryer, L. (2007). Biochemistry. 6th ed. New York, NY 10010: W.H. Freeman and Company. 117-118.</ref>. | DNA Polymerase is an [[Enzyme|enzyme]] which promotes or catalyzes the joining of [[Deoxyribonucleotide|deoxyribonucleotide]] units ([[Phosphodiester bond|Phosphodiester bond]] formation) to form a [[DNA|DNA]] strand. It is essentially involved in [[DNA|DNA]] replication and [[DNA|DNA]] repair. During [[DNA replication|DNA replication]], DNA polymerase requires the presence of a template [[DNA|DNA]] strand which it copies and synthesizes a new [[DNA|DNA]] strand. The newly synthesized [[DNA|DNA]] strand is complementary to the template strand. The catalytic activity of joining the [[Deoxyribonucleotide|deoxyribonucleotide]] requires four precursors which are the deoxynucleoside 5' triphosphates [[DATP|dATP]], [[DGTP|dGTP]], [[DCTP|dCTP]] and [[DTTP|dTTP]]. [[Magnesium|Magnesium]] [[Ions|ions]] are also required for the catalytic activity <ref>Berg, J. Tymoczko, J. Stryer, L. (2007). Biochemistry. 6th ed. New York, NY 10010: W.H. Freeman and Company. 117-118.</ref>. | ||
The DNA polymerase adds nucleotides to the 3' end of the primer<ref>Berg JM, Tymoczko JL, Stryer L. (2002) Biochemistry, 5th Edition, New York: WH Freeman</ref> | The DNA polymerase adds nucleotides to the 3' end of the primer<ref>Berg JM, Tymoczko JL, Stryer L. (2002) Biochemistry, 5th Edition, New York: WH Freeman</ref> | ||
=== References === | === References === | ||
<references /> | <references /> | ||
Revision as of 03:06, 23 November 2018
DNA Polymerase is an enzyme which promotes or catalyzes the joining of deoxyribonucleotide units (Phosphodiester bond formation) to form a DNA strand. It is essentially involved in DNA replication and DNA repair. During DNA replication, DNA polymerase requires the presence of a template DNA strand which it copies and synthesizes a new DNA strand. The newly synthesized DNA strand is complementary to the template strand. The catalytic activity of joining the deoxyribonucleotide requires four precursors which are the deoxynucleoside 5' triphosphates dATP, dGTP, dCTP and dTTP. Magnesium ions are also required for the catalytic activity [1].
The DNA polymerase adds nucleotides to the 3' end of the primer[2]