2D gel electrophoresis: Difference between revisions
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It is a powerful method of separating [[Protein|protein]] along a tubular gel using [[Isoelectric focusing|iso-electric focusing]] and [[ | It is a powerful method of separating [[Protein|protein]] along a tubular gel using [[Isoelectric focusing|iso-electric focusing]] and [[SDS_polyacrylamide-gel_electrophoresis|sodium dodecyl sulphate polyacrylamide gel]]. The sample undergoes [[Isoelectric focusing|iso-electric focusing]] first; producing first dimension. The sample is then placed horizontally on top of [[SDS-PAGE|SDS-PAGE]] and spreads across. The sample then moves vertically down again to yield the second dimension;producing a 2D pattern. So the sample has been separated by virtue of [[Isoelectric point|Iso-electric point]] (pI), when they move horizontally and by virtue of their size; when they move down vertically. | ||
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↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Chapter 3, Page 76. <br> | ↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Chapter 3, Page 76. <br> | ||
Revision as of 11:21, 2 December 2011
It is a powerful method of separating protein along a tubular gel using iso-electric focusing and sodium dodecyl sulphate polyacrylamide gel. The sample undergoes iso-electric focusing first; producing first dimension. The sample is then placed horizontally on top of SDS-PAGE and spreads across. The sample then moves vertically down again to yield the second dimension;producing a 2D pattern. So the sample has been separated by virtue of Iso-electric point (pI), when they move horizontally and by virtue of their size; when they move down vertically.
↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Chapter 3, Page 76.