2D gel electrophoresis: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
It is a powerful method of separating [[Protein|protein]] along a tubular gel using [[Isoelectric focusing|iso-electric focusing]] and [[ | It is a powerful method of separating [[Protein|protein]] along a tubular gel using [[Isoelectric focusing|iso-electric focusing]] and [[SDS polyacrylamide-gel electrophoresis|sodium dodecyl sulphate polyacrylamide gel]]. The sample undergoes [[Isoelectric focusing|iso-electric focusing]] first; producing first dimension. The sample is then placed horizontally on top of [[SDS-PAGE|SDS-PAGE]] and spreads across. The sample then moves vertically down again to yield the second dimension;producing a 2D pattern. So the sample has been separated by virtue of [[Isoelectric point|Iso-electric point]] (pI), when they move horizontally and by virtue of their size; when they move down vertically <ref>Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Chapter 3, Page 76.</ref>. | ||
=== References === | |||
<references /> | |||
Revision as of 14:09, 12 October 2012
It is a powerful method of separating protein along a tubular gel using iso-electric focusing and sodium dodecyl sulphate polyacrylamide gel. The sample undergoes iso-electric focusing first; producing first dimension. The sample is then placed horizontally on top of SDS-PAGE and spreads across. The sample then moves vertically down again to yield the second dimension;producing a 2D pattern. So the sample has been separated by virtue of Iso-electric point (pI), when they move horizontally and by virtue of their size; when they move down vertically [1].
References
- ↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Chapter 3, Page 76.