Reverse transcriptase: Difference between revisions
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Reverse Transcriptase is an [[Enzyme|enzyme]] which copies single stranded [[MRNA|mRNA]] into duplex [[CDNA|cDNA]] (complementary DNA). | Reverse Transcriptase is an [[Enzyme|enzyme]] which copies single stranded [[MRNA|mRNA]] into duplex [[CDNA|cDNA]] (complementary DNA). | ||
Retro viruses have a genome made up of mRNA. They utilise reverse transcriptase to reverse transcribe their mRNA into cDNA. This is so that the viral genome is incorporated into the genome of the host for viral reproduction. | Retro viruses have a genome made up of mRNA. They utilise reverse transcriptase to reverse transcribe their mRNA into cDNA. This is so that the viral genome is incorporated into the genome of the host for viral reproduction. | ||
The single strand of [[MRNA|mRNA]] acts as a template which the reverse transcriptase [[Enzyme|enzyme]] binds to and from which the [[CDNA|cDNA]] is created <ref>Hartl, DL. and Jones EW.(2009:pg439) Genetics: Analysis Of Genes And Genomes, Seventh Edition, London: Jones and Bartlett</ref>. | The single strand of [[MRNA|mRNA]] acts as a template which the reverse transcriptase [[Enzyme|enzyme]] binds to and from which the [[CDNA|cDNA]] is created <ref>Hartl, DL. and Jones EW.(2009:pg439) Genetics: Analysis Of Genes And Genomes, Seventh Edition, London: Jones and Bartlett</ref>. | ||
Revision as of 17:39, 21 October 2012
Reverse Transcriptase is an enzyme which copies single stranded mRNA into duplex cDNA (complementary DNA).
Retro viruses have a genome made up of mRNA. They utilise reverse transcriptase to reverse transcribe their mRNA into cDNA. This is so that the viral genome is incorporated into the genome of the host for viral reproduction.
The single strand of mRNA acts as a template which the reverse transcriptase enzyme binds to and from which the cDNA is created [1].
Reference
- ↑ Hartl, DL. and Jones EW.(2009:pg439) Genetics: Analysis Of Genes And Genomes, Seventh Edition, London: Jones and Bartlett