CDNA

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cDNA stands for complimentary DNA. Reverse transcriptase is used to synthesise cDNA from a single strand of mature mRNA. Reverse transcriptase is used as it operates as a single strand of mRNA generating cDNA by pairing RNA base pairs to complimentary DNA [1]. cDNA is not identical to genomic DNA as it only contains exons, as all of the introns have been removed by splicing to give mature mRNA [2].

References

  1. http://humangenes.org/cdna-complementary-dna 02/12/2015
  2. Hartl and Jones (2009) Genetics Analysis of Genes and Genomes 7th edition, 439, Jones and Bartlett Publishers, Massachusetts. Berg et al (2007) Biochemistry 6th edition, 153, W. H. Freeman and Company, New York.
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