Reporter genes: Difference between revisions
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A reporter [[Gene|gene]] is a gene coding a protein molecule that can be easily assayed. The main use of [[Promoter|promoter]] genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is [[Β-galactosidase|beta-galactosidase.]] In experiments within the lab, reporter genes are usually ligated into a [[Plasmid|plasmid vector]] along with the [[DNA|DNA]] fragments which have different length sequences of DNA upstream of the [[Transcription start site|transcription start site]]. The [[Plasmid|plasmids]] are then seperately transfected into cells, such as cultured ''[[Escherichia coli|E. coli]]''. The cell extract can then be easily assayed to show levels of the reporter enzyme and therefore show how active the promoter is <ref>H. Lodish et al. (2008). Molecular Cell Biology. 8th edition. New York: W.H. Freeman</ref>.<br> | A reporter [[Gene|gene]] is a gene coding a protein molecule that can be easily assayed. The main use of [[Promoter|promoter]] genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is [[Β-galactosidase|beta-galactosidase.]] In experiments within the lab, reporter genes are usually ligated into a [[Plasmid|plasmid vector]] along with the [[DNA|DNA]] fragments which have different length sequences of DNA upstream of the [[Transcription start site|transcription start site]]. The [[Plasmid|plasmids]] are then seperately transfected into cells, such as cultured ''[[Escherichia coli|E. coli]]''. The cell extract can then be easily assayed to show levels of the reporter [[enzyme|enzyme]] and therefore show how active the [[promoter|promoter]] is <ref>H. Lodish et al. (2008). Molecular Cell Biology. 8th edition. New York: W.H. Freeman</ref>.<br> | ||
=== References === | === References === | ||
Revision as of 20:42, 24 October 2012
A reporter gene is a gene coding a protein molecule that can be easily assayed. The main use of promoter genes is to show whether a promoter the gene is linked to, is activated or not. A very common reporter gene is beta-galactosidase. In experiments within the lab, reporter genes are usually ligated into a plasmid vector along with the DNA fragments which have different length sequences of DNA upstream of the transcription start site. The plasmids are then seperately transfected into cells, such as cultured E. coli. The cell extract can then be easily assayed to show levels of the reporter enzyme and therefore show how active the promoter is [1].
References
- ↑ H. Lodish et al. (2008). Molecular Cell Biology. 8th edition. New York: W.H. Freeman