Restriction enzyme: Difference between revisions
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*[[EcoRI|''Eco''RI]]- recognines the sequence GAATTC | *[[EcoRI|''Eco''RI]]- recognines the sequence GAATTC | ||
*[[BamHI|''Bam''HI]]- recognises the sequence GGATCC | *[[BamHI|''Bam''HI]]- recognises the sequence GGATCC | ||
*[[HaeIII|''Hae''III]]- recognises GGCC | *[[HaeIII|''Hae''III]]- recognises GGCC | ||
*''<u>Hpal</u>'' - recognises GTTAAC | *''<u>Hpal</u>'' - recognises GTTAAC | ||
*''<u>HindIII</u>'' - recognises AAGCTT | *''<u>HindIII</u>'' - recognises AAGCTT | ||
*''<u>PstI</u>'' - recognises CTGCAG | *''<u>PstI</u>'' - recognises CTGCAG | ||
*<u>''Sau3A''</u> | |||
Both ''Eco''RI and ''Bam''HI produce fragments with [[‘sticky’ ends|staggered ends]], whilst ''Hae''III produces a DNA fragment with [[Blunt ends|blunt ends]] i.e. there is no overhang. | Both ''Eco''RI and ''Bam''HI produce fragments with [[‘sticky’ ends|staggered ends]], whilst ''Hae''III produces a DNA fragment with [[Blunt ends|blunt ends]] i.e. there is no overhang. | ||
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Also see [[Restriction endonucleases|Restriction endonucleases]] | Also see [[Restriction endonucleases|Restriction endonucleases]] | ||
Revision as of 16:39, 25 October 2012
Restriction Endonucleases cut DNA at a specific sequence, normally 4, 6 or 8 bases long. They recognise specific palindromic base sequences in DNA and are used to selectively cut DNA into defined fragments at sites known as 'restriction sites'. Different restriction nucleases are obtained and purified from different species of bacteria. These enzymes are made in bacteria to degrade viral DNA. Cutting DNA with restriction enzymes can produce fragments with either blunt or sticky ends. Different restriction enzymes recognise different DNA sequences.
Examples of restriction endonucleases are:
- EcoRI- recognines the sequence GAATTC
- BamHI- recognises the sequence GGATCC
- HaeIII- recognises GGCC
- Hpal - recognises GTTAAC
- HindIII - recognises AAGCTT
- PstI - recognises CTGCAG
- Sau3A
Both EcoRI and BamHI produce fragments with staggered ends, whilst HaeIII produces a DNA fragment with blunt ends i.e. there is no overhang.
Also see Restriction endonucleases