Primary antibody: Difference between revisions
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Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies. Alberts et al | Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies. (Alberts et al 2002 ''Molecular Biology of the cell'' 5th , (9)588-589) | ||
Revision as of 21:30, 21 October 2013
Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies. (Alberts et al 2002 Molecular Biology of the cell 5th , (9)588-589)