Restriction enzymes
Restriction Enzymes, or Restriction Endonucleases cut DNA into smaller fragments [1].
Different restriction enzymes recognise different squences, normally between 4 to 8 nucleotides in length. Some restriction enzymes produce staggered or 'sticky' ends while others leave blunt ends[2]. Examples of restriction enzymes that produce staggered ends are EcoR1 and Hind111[3]. Hpal is an example of a restriction enzyme that forms blunt ends [4].
Restriction Enzymes are often used to create recombinant DNA molecules. See Recombinant DNA Technology.
References
- ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science
- ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science
- ↑ Dryden, D.T.F., Loenen, W.A.M., Murray, N.E., Raleigh, E.A., Wilson, G.G., 2013. Highlights of the DNA Cutters: a short history of restriction enzymes. Nucleic Acids Research, 42(1), pp 3-19.
- ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science