SDS-PAGE
See SDS polyacrylamide-gel electrophoresis
SDS (Sodium Dodecyl Sulfate), a negatively charged substance or anionic surfactant with a sulfate group attached, allows proteins of all charges to travel down the polyacrylamide to the positive electrode [1]. This SDS will bind to proteins and disrupt non-covalent bonds, which causes denaturation in proteins. Once the protein is bound to the SDS molecule it will become negatively charged.
References
- ↑ Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/