Immunolabelling

Immunolebelling is a technique used to detect proteins this could in a cell or in an expertiment such as Western Blotting. An antibody that will recongise that your desired protein is added, in case of a cell a method to get the antibody into the cell must be deveopled. The antibody (primary antibody) will specifically bind to the antigen. In a Western Blottting experiment this take about an one hour and half on a moving plate. Then you add a secondary antibody which recongises the primary antibody as a antigen. The secondary antibody is conjugated with fluorescent marker. So the location of your protein can be detected using this technique .