SDS-PAGE
See SDS polyacrylamide-gel electrophoresis
SDS (Sodium Dodecyl Sulfate) is a negatively charged substance/anionic surfactant that allows proteins of all charges to travel down the polyacrylamide to the positive electrode [1]. i.e. This SDS will bind to proteins and disrupt non-covalent bonds. This causes denaturation in proteins. SDS molecule has a sulfate group attacted, a protein will then become negatively charged when it is binded to SDS molecule.
References
- ↑ Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/