SDS-PAGE

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This separates proteins by virtue of mass. This is done under denaturing conditions as SDS denatures protein. The SDS also adds negative chage to the proteins which would enable them to move towards the positive end of the electrode. It gives a high degree of resolution.



↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Page 75.
↑ Berg, J. Stryer, L. Tymoczko, J. (2011) Biochemistry, 7th Edition, New York: W.H Freeman and Company. Page 80.