DNA Sequencing

The advent of techniques for the rapid sequencing of DNA has led to many major advances in molecular biology. Several methods have now been developed for determining the nucleotide sequence of DNA, but the Sanger (“dideoxy”) method offers significant advantages in terms of rapidity and simplicity of protocol. It is based upon the use of deoxynucleotide analogues that are randomly incorporated into a growing DNA strand to give specific chain termination.