User talk:150010450

It is a powerful method of spreading protein along a tubular gel using iso-electric focusing and sodium dodecyl sulphate polyacrylamide gel. the sample undergoes iso-electric focusing first, producing first dimension. the sample is then placed horizontally on top of SDS-PAGE and spreads across. the sample then moves vertically down again to yield the second dimension; producing a 2D pattern. So the sample has been separated by  virtue of Iso-electric point (pl), when they move horizontally and by virtue of their size; when they move down vertically.