Template strand

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DNA Template strand (also known as the aniti-sense strand), is one of the strands of DNA after being unwound by Helicase- which happens during transcription of a gene. RNA polymerase binds to the template strand using it as a 'template' during transcription, this produces a complementary pre-mRNA molecule within the nucleus from free RNA nucleotides binding by complementary base pairing to the bases on the template strand. Splicing will then take place in order to remove any non-coding regions of DNA (occurs only in Eukaryotes) to give the final mRNA strand, which leaves the nucleus through a pore and joins to a ribosome where translation of this mRNA will occur.

This mRNA can then be translated by ribosomes forming a sequence of amino acids [1].

References 

  1. Lodish H., Kaiser CA., Bretscher A., Amon A., Berk A., Krieger M., Ploegh H and Scott MP (2012) Molecular Cell Biology, 7th edition, New York: WH FreemanfckLRpage:124