Closed complex
The closed complex refers to a state in Prokaryotic gene expression where by the RNA Holoenzyme (RNA polymerase with the associated sigma factor) is bound to the DNA[1]. The enzyme binds to the -35 and -10 promoter regions these are hexamers- sequence of six base pairs [2]. After around 10 base transcriptions the sigma factor disassociates from the RNA and elongation continues. This state is referred to as the open complex [3].
See Simon Whitehall’s CMB2001 Lecture 1 (18/19) for further detail.
Referances
- ↑ B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.
- ↑ A Kanhere, M Bansal. Structural properties of promoters: similarities and differences between prokaryotes and eukaryotes. Nucleic Acid Research 2005; 33(10): 3165-3175.
- ↑ B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.