Recombinant DNA Technology
Introduction
Recombinant DNA molecules are new artificial DNA strands that are produced by combining two unrelated (non-homologous) genes, for example: hybrid of E. coli plasmid with human insulin gene. It is possible to join two unrelated genes from different species because all organisms in the world share the same DNA makeup (nitrogen bases, sugar, and phosphate backbone) and only differ in the sequence[1] . So one strand of DNA can complement the other strand according to Chargaff's rules.
Molecular Tools for making Recombinant DNA
References
- ↑ Glick, B.R., Pasternak, J.J. and Patten, C.L. (2010) Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th edition, United States: America Society for Microbiology.
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