Dna ligase

DNA ligase is an enzyme used for the ligation of blunt ended and sticky ended recombinant fragments or Okazaki fragments. It works by repairing breaks in the sugar phosphate backbone in DNA by creating a covalent bond between adjoining nucleotides (between the 3’hydroxyl group of one DNA molecule with the 5’phosphoryl group of another).
In eukaryotes DNA ligase I family members play the major role.The enzyme has a fundamental role in genetic engineering such as recombinant plasmid formation or Polymerase Chain Reaction.