Mobile phase

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The mobile phase is a liquid, gas or supercritical fluid used in chromatography.

The role of the mobile phase is to act as a solvent, carrying the mixture over the stationary phase through the chromatography column. Constituents of the mixture will then be able to be separated based on their affinity with the stationary and mobile phases. A compound with a lower affinity for the stationary phase will have a lower retention time and leave the column quicker.

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