Coomassie blue: Difference between revisions

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 Coomassie Brilliant Blue G-250 is a triphenylmethane which is commonly used as a dye to visuallise protein bands during PCR. 
Coomassie Brilliant Blue G-250<ref>http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf</ref> is a triphenylmethane which is commonly used as a dye to visuallise [[Protein|protein]] bands.&nbsp;  


Methology of staining the polyacrylamide with the dye is as follows
Methology of staining the [[SDS-PAGE|polyacrylamide]] with the dye is as follows:


#If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water.
#If the gel contains [[SDS|SDS]] then wash it 3 times for 5 minuetes each in deionised water.  
#Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minuetes however will be at there peak of intensity within 1 hour.
#Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minutes however will be at there peak of intensity within 1 hour.  
#Repeat stage 1 to intesify the protein bands.
#Repeat stage 1 to intesify the protein bands.


=== References  ===


<references />


http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf
<br>
 
<br>

Latest revision as of 02:27, 24 October 2014

Coomassie Brilliant Blue G-250[1] is a triphenylmethane which is commonly used as a dye to visuallise protein bands. 

Methology of staining the polyacrylamide with the dye is as follows:

  1. If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water.
  2. Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minutes however will be at there peak of intensity within 1 hour.
  3. Repeat stage 1 to intesify the protein bands.

References