Chromosome walking: Difference between revisions
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Created page with " Chromosome walking is technique that can be used to construct a clone contig by identifying overlapping fragments of cloned DNA.<ref>http://www.ncbi.nlm.nih.gov/books/..." |
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[[Chromosome|Chromosome]] walking is technique that can be used to construct a [[clone|clone]] contig by identifying overlapping fragments of cloned [[DNA|DNA]].<ref>http://www.ncbi.nlm.nih.gov/books/NBK21117/</ref> This method is mainly used when [[sequencing|sequence]] of [[genetic map|gene]] is known but no clone gene avaliable. <sup></sup> | |||
Example: | Example: | ||
Latest revision as of 06:19, 24 November 2015
Chromosome walking is technique that can be used to construct a clone contig by identifying overlapping fragments of cloned DNA.[1] This method is mainly used when sequence of gene is known but no clone gene avaliable.
Example:
ATCGTAGCTAGCGATTTC
ATTTCGATTATCGATTTCGATGCTA
CGATGCTAGCCGATTTAGCTAGC
TTAGCTAGCTACGCGATCGATCGAGAGAG
ATCGTAGCTAGCG ATTTC GATTATCGATTT CGATGCTA GCCGAT TTAGCTAGC TACGCGATCGATCGAGAGAG