Chromosome walking

From The School of Biomedical Sciences Wiki
Jump to navigation Jump to search

Chromosome walking is technique that can be used to construct a clone contig by identifying overlapping fragments of cloned DNA.[1] This method is mainly used when sequence of gene is known but no clone gene avaliable. 

Example:

ATCGTAGCTAGCGATTTC
                             ATTTCGATTATCGATTTCGATGCTA
                                                               CGATGCTAGCCGATTTAGCTAGC
                                                                                              TTAGCTAGCTACGCGATCGATCGAGAGAG
  
ATCGTAGCTAGCG ATTTC GATTATCGATTT CGATGCTA GCCGAT TTAGCTAGC TACGCGATCGATCGAGAGAG
 

Reference: 

  1. ↑ http://www.ncbi.nlm.nih.gov/books/NBK21117/
Retrieved from "https://teaching.ncl.ac.uk/bms/wiki//index.php?title=Chromosome_walking&oldid=14297"