Sticky ends: Difference between revisions
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Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | ||
[[BamHI|BamHI]] | *[[BamHI|BamHI]] | ||
*[[BamHI|BamHI]][[BaI228I|BaI228I]] | |||
*[[BaI228I|BaI228I]][[EcoRI|EcoRI]] <ref name="null">http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation</ref><br> | |||
=== References === | === References === | ||
<references /><br> | <references /><br> | ||
Revision as of 13:14, 30 November 2015
Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1]. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends.
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: