RFLP

Restriction fragment length polymorphisms are a type of genetic identifiers used in the study of traits and comparison of allelic restriction sites. RFLPs develop due to the mutations that occur in the sequences that code restriction sites; thus the mutations either result in the destruction or creation of restriction sites. The consequence of such mutations is that when a restriction enzyme is used to cut the DNA sequence, where there has been destructive mutation the DNA sequence is not ligated and where there has been creation of restriction sites the DNA is ligated. This all leads to varying lengths of DNA fragments which separate on gel electrophoresis. RFLPs are used for the analysis of traits and allelic mutations .