Gel electrophoresis

From The School of Biomedical Sciences Wiki
Jump to: navigation, search
Example of Gel Electrophoresis

Molecules can be separated by many different methods. One of them is gel electrophoresis. Gel electrophoresis is used for purification of proteins. In general, gel electrophoresis is based on the movement of molecules, such as RNA and DNA, under an electric field. The main idea of the separation is the movement of these molecules through small pores. The electric field created all over the gel enables the molecules to move from negative to the positive electrodes. DNA is negatively charged, therefore moves towards the positive electrode. Large molecules move slowy and can easily get attached to the pores, this prevents them moving from one side of the gel to the other. Whereas, small molecules move more rapidly and they can also move further than the big molecules do. Hence this mechanism allows the seperation of molecules due to their their size. Gel electrophoresis also allows the separation of molecules based on how coiled or linear they are. For example, supercoiled plasmids move more rapidly through the gel than linear plasmids [1][2] .

References

  1. Berg.J, Tymoczko J., and Stryer L., (2012) Biochemistry, Seventh Edition, New York: WH Freeman
  2. http://www.whoi.edu/cms/images/tgoepfert/2004/11/IMG_3426_3562.jpg


Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox