Sticky ends: Difference between revisions
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Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>.<br> | Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>. Restriction enzymes can also symmetrically cleave DNA strands to form [[Blunt ends|blunt ends]]. In [[Recombinant_DNA_Technology|recombinant DNA techniques]] when producing a recombinant plasmid the same restriction endonuclease is used to cut both the plasmid DNA and the gene of interest so that they have complimentary sticky ends that will anneal to produce a recombinant plasmid.<br> | ||
Some examples of Restriction endonucleases that cut to create 'sticky ends' are <ref>http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation</ref>:<br> | |||
: | |||
*[[BamHI|BamHI]] | |||
*[[BaI228I|BaI228I]] | |||
*[[EcoRI|EcoRI]] | |||
=== References === | |||
<references /><br> | |||
Latest revision as of 12:57, 25 October 2017
Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1]. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends. In recombinant DNA techniques when producing a recombinant plasmid the same restriction endonuclease is used to cut both the plasmid DNA and the gene of interest so that they have complimentary sticky ends that will anneal to produce a recombinant plasmid.
Some examples of Restriction endonucleases that cut to create 'sticky ends' are [2]: