Coomassie blue: Difference between revisions
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Visualise protein bands in PCR? |
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Coomassie Brilliant Blue G-250<ref>http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf</ref> is a triphenylmethane which is commonly used as a dye to visuallise [[ | Coomassie Brilliant Blue G-250<ref>http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf</ref> is a triphenylmethane which is commonly used as a dye to visuallise [[Protein|protein]] bands. | ||
Methology of staining the polyacrylamide with the dye is as follows: | Methology of staining the [[SDS-PAGE|polyacrylamide]] with the dye is as follows: | ||
#If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water. | #If the gel contains [[SDS|SDS]] then wash it 3 times for 5 minuetes each in deionised water. | ||
#Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 | #Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minutes however will be at there peak of intensity within 1 hour. | ||
#Repeat stage 1 to intesify the protein bands. | #Repeat stage 1 to intesify the protein bands. | ||
=== References === | === References === | ||
<references /> | <references /> | ||
<br> | <br> | ||
<br> | <br> | ||
Latest revision as of 02:27, 24 October 2014
Coomassie Brilliant Blue G-250[1] is a triphenylmethane which is commonly used as a dye to visuallise protein bands.
Methology of staining the polyacrylamide with the dye is as follows:
- If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water.
- Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minutes however will be at there peak of intensity within 1 hour.
- Repeat stage 1 to intesify the protein bands.
References