Genetic mutation: Difference between revisions
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'''Genetic mutation- '''this term indicates | '''Genetic mutation- '''this term indicates an alteration to base sequence in [[DNA|DNA]] of an organism which results in a mutant [[Allele|allele]] of a [[Gene|gene]] i.e. an allele that occurs in less than 1% of population. The change is heritable and can be passed on to offspring if fixed into the parental [[Genome|genome]]. Mutations can vary depending on the type of genomic alteration: | ||
*addition | *addition | ||
*deletion | *deletion | ||
*substitution | *substitution | ||
*rearrangement of bases. | *rearrangement of bases. | ||
To investigate the events leading to mutation we can look at mutagenesis process | To investigate the events leading to mutation we can look at the 3 types of mutagenesis process: | ||
*spontaneous (no mutation causing agent) | *spontaneous (no mutation causing agent) | ||
*induced (a foreign genome introduced e.g.in the laboratory) | *induced (a foreign genome introduced e.g.in the laboratory) | ||
*directed (in vitro change to genome). | *directed (''in vitro'' change to the genome). | ||
We can calculate the rate of mutation occurance using an equation from Poisson distribution: | We can calculate the rate of mutation occurance using an equation from Poisson distribution: | ||
'''P<sub>0</sub>=e<sup>-mN</sup>'''<sup></sup> | '''P<sub>0</sub>=e<sup>-mN</sup>'''<sup></sup> | ||
where P<sub>0</sub> is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate <ref>Genetics, 1987, Geoffrey Zubay</ref><ref>Encyclopedia of Genetics,Volume 3, 2002, S.Brenner,J.H. Miller</ref>. | where P<sub>0</sub> is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate <ref>Genetics, 1987, Geoffrey Zubay</ref><ref>Encyclopedia of Genetics,Volume 3, 2002, S.Brenner,J.H. Miller</ref>. | ||
<references / | === References === | ||
<references /> | |||
<br> | <br> | ||
Latest revision as of 09:34, 18 November 2017
Genetic mutation- this term indicates an alteration to base sequence in DNA of an organism which results in a mutant allele of a gene i.e. an allele that occurs in less than 1% of population. The change is heritable and can be passed on to offspring if fixed into the parental genome. Mutations can vary depending on the type of genomic alteration:
- addition
- deletion
- substitution
- rearrangement of bases.
To investigate the events leading to mutation we can look at the 3 types of mutagenesis process:
- spontaneous (no mutation causing agent)
- induced (a foreign genome introduced e.g.in the laboratory)
- directed (in vitro change to the genome).
We can calculate the rate of mutation occurance using an equation from Poisson distribution:
P0=e-mN
where P0 is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate [1][2].
References