Bacterial artificial chromosome: Difference between revisions
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A Bacterial Artificial Chromosome (BAC) is larger than a regular [[Plasmid|plasmid]]. They are often used in PCR ([[Polymerase Chain Reaction|Polymerase Chain Reaction]]) as they can take up larger inserts than a regular plasmid. [[DNA|DNA]] fragments of around 100-200 kb can be inserted into a BAC. A BAC is similar to an [[F' Plasmid|F' Plasmid]]<ref>Molecular Biology of the Cell, Alberts et al, 5th Edition (2007) Garland Science, New York, Chapter 8, Page 541</ref>. | |||
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Latest revision as of 16:21, 4 December 2017
A Bacterial Artificial Chromosome (BAC) is larger than a regular plasmid. They are often used in PCR (Polymerase Chain Reaction) as they can take up larger inserts than a regular plasmid. DNA fragments of around 100-200 kb can be inserted into a BAC. A BAC is similar to an F' Plasmid[1].
References
- ↑ Molecular Biology of the Cell, Alberts et al, 5th Edition (2007) Garland Science, New York, Chapter 8, Page 541