Sticky ends: Difference between revisions
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Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends.<br> | Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>. Restriction enzymes can also symmetrically cleave DNA strands to form [[Blunt ends|blunt ends]].<br> | ||
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | ||
Revision as of 20:10, 29 November 2015
Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1]. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends.
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: