Electroporation: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[File coli-ed01.jpg|frame|right|Figure 1: E. coli (a common prokaryotic model organism into which recombinant plasmds are inserted)]] Electroporation is a method used to insert a recombinant [[Plasmid|plasmid]] into a [[Bacterial cell|bacterial cell]] using high voltage electrical pulses<ref>http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html</ref>. | [[File:coli-ed01.jpg|frame|right|Figure 1: E. coli (a common prokaryotic model organism into which recombinant plasmds are inserted)]] Electroporation is a method used to insert a recombinant [[Plasmid|plasmid]] into a [[Bacterial cell|bacterial cell]] using high voltage electrical pulses<ref>http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html</ref>. | ||
In molecular biology, electroporation is commonly used for cell [[Transfection|transfection]] or [[Transformation|transformation]] of non-viral [[DNA|DNA]] transfer of [[Bacteria|bacteria]], [[Yeast|yeast]], and plant plastids. Electroporation is also highly effective for the introduction of foreign [[Genes|genes]] into tissue culture cells, especially mammalian cells<ref>Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/</ref>. | In molecular biology, electroporation is commonly used for cell [[Transfection|transfection]] or [[Transformation|transformation]] of non-viral [[DNA|DNA]] transfer of [[Bacteria|bacteria]], [[Yeast|yeast]], and plant plastids. Electroporation is also highly effective for the introduction of foreign [[Genes|genes]] into tissue culture cells, especially mammalian cells<ref>Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/</ref>. | ||
Revision as of 17:57, 16 November 2017
Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses[1].
In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells[2].
Example of electroporation:
E. coli plasmid insertion with CaCl2 on ice with heat shock.
References
- ↑ http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html
- ↑ Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/