RNA polymerase II: Difference between revisions

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Found in Eukaryotic cells, RNA polymerase II is used&nbsp;to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs ([[snoRNA|snoRNA]])&nbsp;and the majority of the micro RNAs (miRNA)<ref>Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74</ref>. RNA polymerase II cannot transcribe DNA on its own: the many&nbsp;elements that make up the [[Pre_Initiation_Complex|pre-initiation complex]] (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription<ref>Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595</ref>. The PIC is made up of Transcription Factors: [[TFIID|TFIID]], [[TFIIA|TFIIA]], [[TFIIB|TFIIB]], [[TFIIF|TFIIF]], [[TFIIE|TFIIE]], [[TFIIH|TFIIH]]<ref>D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.</ref>.  
Found in [[Eukaryotic_cells|eukaryotic cells]], RNA polymerase II is used&nbsp;to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs ([[SnoRNA|snoRNA]])&nbsp;and the majority of the micro RNAs (miRNA)<ref>Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74</ref>. RNA polymerase II cannot transcribe [[DNA|DNA]] on its own: the many&nbsp;elements that make up the [[Pre Initiation Complex|pre-initiation complex]] (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription<ref>Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595</ref>. The PIC is made up of Transcription Factors: [[TFIID|TFIID]], [[TFIIA|TFIIA]], [[TFIIB|TFIIB]], [[TFIIF|TFIIF]], [[TFIIE|TFIIE]], [[TFIIH|TFIIH]]<ref>D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.</ref>.<br>


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=== References ===
 
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Revision as of 21:22, 26 November 2011

Found in eukaryotic cells, RNA polymerase II is used to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs (snoRNA) and the majority of the micro RNAs (miRNA)[1]. RNA polymerase II cannot transcribe DNA on its own: the many elements that make up the pre-initiation complex (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription[2]. The PIC is made up of Transcription Factors: TFIID, TFIIA, TFIIB, TFIIF, TFIIE, TFIIH[3].

References

  1. ↑ Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74
  2. ↑ Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595
  3. ↑ D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.
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