Sticky ends: Difference between revisions
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Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>.<br> | Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>.<br> | ||
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | ||
BamHI,BaI228I and EcoRI <ref name="null">http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation</ref><br> | [[BamHI|BamHI]], [[BaI228I|BaI228I]] and [[EcoRI|EcoRI]] <ref name="null">http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation</ref><br> | ||
=== References === | |||
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Revision as of 02:07, 24 October 2014
Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1].
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: