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Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>. Restriction enzymes can also symmetrically cleave DNA strands to form [[Blunt ends|blunt ends]].<br> | Sticky ends are fragments of [[DNA|DNA]] where one strand, after being cleaved by [[Restriction enzymes|restriction enzymes]], is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free [[Nucleotides|nucleotides]] <ref>http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html</ref>. Restriction enzymes can also symmetrically cleave DNA strands to form [[Blunt ends|blunt ends]].<br> | ||
Some examples of Restriction endonucleases that cut to create 'sticky ends' are: | Some examples of Restriction endonucleases that cut to create 'sticky ends' are <ref>http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation</ref><br> | ||
: | |||
*[[BamHI|BamHI]] | *[[BamHI|BamHI]] | ||
*[[BaI228I|BaI228I]] | *[[BaI228I|BaI228I]] | ||
*[[EcoRI|EcoRI]] | *[[EcoRI|EcoRI]] | ||
=== References === | === References === | ||
<references /><br> | <references /><br> | ||
Revision as of 13:15, 30 November 2015
Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1]. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends.
Some examples of Restriction endonucleases that cut to create 'sticky ends' are [2]