Modifying enzymes: Difference between revisions
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Modifying enzymes remove or add specific chemical groups. For example, [[Alkaline phosphatase|Alkaline phosphatase]] (from [[E. coli|''E. coli'']], calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a [[DNA|DNA]] molecule <ref>T.A.Brown (2010). Gene cloning &amp;amp; DNA analysis: an introduction. 6th ed. Hoboken : Wiley-Blackwell. p49-50</ref>.<br> | Modifying [[Enzyme|enzymes]] remove or add specific chemical groups. For example, [[Alkaline phosphatase|Alkaline phosphatase]] (from [[E. coli|''E. coli'']], calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a [[DNA|DNA]] [[molecule|molecule]] <ref>T.A.Brown (2010). Gene cloning &amp;amp;amp; DNA analysis: an introduction. 6th ed. Hoboken : Wiley-Blackwell. p49-50</ref>.<br> | ||
Modifying enzymes are useful to protect the DNA from being cleaved by a restriction enzyme | Modifying enzymes are useful to protect the DNA from being cleaved by a [[restriction enzyme|restriction enzyme]] <ref>http://www.reference.md/files/D015/mD015280.html</ref>. | ||
=== References === | === References === | ||
<references /> | <references /> | ||
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Revision as of 14:57, 21 October 2016
Modifying enzymes remove or add specific chemical groups. For example, Alkaline phosphatase (from E. coli, calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a DNA molecule [1].
Modifying enzymes are useful to protect the DNA from being cleaved by a restriction enzyme [2].
References
- ↑ T.A.Brown (2010). Gene cloning &amp;amp; DNA analysis: an introduction. 6th ed. Hoboken : Wiley-Blackwell. p49-50
- ↑ http://www.reference.md/files/D015/mD015280.html