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The closed complex refers to a state in Prokaryotic gene expression where by the RNA [[Holoenzyme|Holoenzyme]] (RNA polymerase with the associated sigma factor) is bound to the DNA<ref>B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.</ref>. The enzyme binds to the -35 and -10 promoter regions these are hexamers- sequence of six base pairs <ref>A Kanhere, M Bansal. Structural properties of promoters: similarities and differences between prokaryotes and eukaryotes. Nucleic Acid Research 2005; 33(10): 3165-3175.</ref>. After around 10 base transcriptions the sigma factor disassociates from the RNA and elongation continues. This state is referred to as the [[Open complex|open complex]]&nbsp;<ref>B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.</ref>.  
The closed complex refers to a state in [[Prokaryotic|prokaryotic]] [[gene expression|gene expression]] whereby the [[RNA|RNA]] [[Holoenzyme|Holoenzyme]] ([[RNA polymerase|RNA polymerase]] with the associated [[Sigma_factor|sigma factor]]) is bound to the [[DNA|DNA]]<ref>B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.</ref>. The enzyme binds to the -35 and -10 promoter regions these are hexamers- sequences of six [[Base|base]] pairs<ref>A Kanhere, M Bansal. Structural properties of promoters: similarities and differences between prokaryotes and eukaryotes. Nucleic Acid Research 2005; 33(10): 3165-3175.</ref>. After around 10 base transcriptions the sigma factor disassociates from the RNA and elongation continues. This state is referred to as the [[Open complex|open complex]]<ref>B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.</ref>.  


See Simon Whitehall’s CMB2001 Lecture 1 (18/19) for further detail.
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Latest revision as of 07:17, 20 October 2018

The closed complex refers to a state in prokaryotic gene expression whereby the RNA Holoenzyme (RNA polymerase with the associated sigma factor) is bound to the DNA[1]. The enzyme binds to the -35 and -10 promoter regions these are hexamers- sequences of six base pairs[2]. After around 10 base transcriptions the sigma factor disassociates from the RNA and elongation continues. This state is referred to as the open complex[3].

References

  1. ↑ B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.
  2. ↑ A Kanhere, M Bansal. Structural properties of promoters: similarities and differences between prokaryotes and eukaryotes. Nucleic Acid Research 2005; 33(10): 3165-3175.
  3. ↑ B Alberts, A Johnson, J Lewis, D Morgan, M Raff, K Roberts, P Walter. Molecular Biology of The Cell. 6th Ed. Abingdon: Garland Science, Taylor and Francis Group, LLC. 2015.
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